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. Author manuscript; available in PMC: 2015 Sep 15.
Published in final edited form as: Cancer Res. 2014 Jul 24;74(18):5195–5205. doi: 10.1158/0008-5472.CAN-14-0697

Figure 2. Combined Ad5Δ24 and GD2.CAR-T cells have superior proapoptotic effect on NB cells in vitro.

Figure 2

(A) Antitumor activity of either monotherapy or combined therapy was assessed by in vitro co-culture assay. These experiments were conducted by using 3 NB cell lines (CHLA-255, IMR-32 and SKNLP) and 6 different preparations of T cells. Data are presented as mean ± SEM. * P < 0.05; ** P < 0.01 by Student’s t test. (B) Sequential confocal microscope imaging of co-culture experiments using GFP+ CHLA-255 cells. Apoptotic CHLA-255 cells binding Annexin-V appear in orange. Data are representative of 2 independent experiments. (C) and (D) Activated caspases in CHLA-255 cells were quantified by flow cytometry and indicated as FAM+/PI− cells at time 0 and after 2 and 4 hrs of culture. Results are mean ± SEM from 7 different preparations of T cells. * P < 0.05 by ANOVA with Bonferroni correction.