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. Author manuscript; available in PMC: 2015 Oct 21.
Published in final edited form as: Lab Chip. 2014 Oct 21;14(20):3925–3936. doi: 10.1039/c4lc00688g

Fig. 4.

Fig. 4

Regulation of hypercontraction via the RhoA/ROCK2 pathway. (A) The contractile response of the IL-13 induced asthmatic phenotype pretreated with 10 μM HA1077. n=15 films, 3 chips for the healthy phenotype; 4 chips, n=23 films for the IL-13 induced asthmatic phenotype; n=12 films, 2 chips for IL-13 tissue pretreated with HA1077. Data points represent mean ± standard error. The ACh dose response was plotted versus concentration denoting statistical significance at each dose, * indicates p<0.05 relative to IL-13 tissue pretreated with HA1077 and # indicates p<0.05 relative to both healthy phenotypic tissues and the IL-13 induced asthmatic phenotypic tissues pretreated with HA1077. (B) For the healthy and the IL-13 induced asthmatic phenotypes, relaxation was quantified in response to HA1077. Stress is measured from the basal tone. n=12 films for the healthy phenotype, 2 chips; n=12 films, 2 chips for the IL-13 induced asthmatic phenotype. Data points represent mean ± standard error. (C) The magnitude of relaxation in response to HA1077 was quantified measuring the change in stress between the basal tone and the tone after the administration of 100 μM HA1077. This response was compared to the relaxation induced in response to 1mM ISO seen in Fig. 2B. * and # indicate p<0.05. (D) Healthy and the IL-13 induced asthmatic phenotypes were relaxed from the basal tone using ISO and HA1077 in series. Stress is measured from the basal tone. n=10 films, 2 chips for the healthy phenotype; n=11 films, 2 chips for the IL-13 induced asthmatic phenotype. Data points represent mean ± standard error. (E) Tissues were constricted using ACh, then relaxed using ATO, ISO, and HA1077 in series. Stress is measured from the basal tone. n=11 films for the healthy phenotype, 2 chips; n=12 films, 2 chips for the IL-13 induced asthmatic phenotype. Data points represent mean ± standard error. (F) The relaxation was quantified by measuring the change in stress between the basal tone and the tone after administration of 100 μM HA1077. * indicates p<0.05 between non-constricted and constricted tissues within a condition. # indicates p<0.05 of the non-constricted/constricted tissues between the healthy and IL-13 conditions.