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. Author manuscript; available in PMC: 2015 Sep 12.
Published in final edited form as: Biochem Biophys Res Commun. 2014 Aug 22;452(1):112–117. doi: 10.1016/j.bbrc.2014.08.057

Figure 1. Ascorbate blocks high glucose-induced pericyte apoptosis.

Figure 1

Panel A: Cells were cultured for 6-7 days at either 5 mM (circles) or 25 mM (squares) glucose with daily addition of the indicated ascorbate concentration, followed by cell rinsing and harvesting for assay of intracellular ascorbate (Panel A) or cleaved caspase 3 (Panel B). Results in each panel are from 6 separate experiments. An “*” indicates p < 0.05 compared to zero ascorbate at the same glucose concentration. An “**” indicates p < 0.05 compared to the value at 25 mM glucose and zero ascorbate. Panel C: Pericytes cultured for 6-7 days at either 5 mM (circles) or 25 mM (squares) glucose were treated during the last 24 hours of culture with the indicated concentrations of FPS-ZM1 and taken for assay of cleaved caspase 3. Results are shown from 5 experiments, with an “*” indicating p < 0.05 compared to the sample cultured at 25 mM glucose alone.