Figure 1. K72 is a SUMOylation site that controls BR-induced CES nuclear body localization.

(a) Fluorescence microscopic images of representative stomata of 14-day-old cpd × 35S:CES^wt-YFP/32 seedlings either untreated (upper panel) or treated with 1 μM BL for 2 h (lower panel). Scale bars: filter = 5 μm, magnified = 2 μm. (b) Structure of the CES protein with the SUMOylation motif underlined. Target lysine in red. (c) In vitro SUMOylation assays using the heterodimeric SUMO activating enzyme E1 and SUMO conjugating enzyme E2, SUM1 and SUM2 as modifiers, and assessing affinity purified, recombinant GST-CES^wt and GST-CES^K72R as substrate proteins. (d) Representative fluorescence microscopic images of stomata of 14-day-old plants of 35S:CES^wt-YFP (line 32, upper two rows) or 35S:CES^K72R-YFP (line 411, lower two rows) either untreated (upper row) or treated with 1 μM Brz for 24 h followed by 1 μM BL for 2 h (lower row).