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. Author manuscript; available in PMC: 2015 Feb 19.
Published in final edited form as: Nat Commun. 2014 Aug 19;5:4687. doi: 10.1038/ncomms5687

Figure 4. CDPKs phosphorylate CES.

Figure 4

(a) Sequence of the extended SUMOylation motif. The simple 1 (basic-XX-S/T) and simple 2 (S-X-R) CDPK recognition sites are highlighted. K72 in red, S75 and S77 in blue. (b) Fluorescence microscopic images of hypocotyls of 14-days-old seedlings of 35S:CESwt-YFP/32 (upper panel) or 35S:CESS75E+S77E-YFP/632 (lower panel), untreated or treated with 300 μM triflouperazine (TFP) for 1.5 h. Scale bars: CESwt-YFP: filter = 20 μm, magnified = 5 μm; CESS75E+S77E-YFP: filter = 20 μm, magnified = 5 μm. (c) In vitro kinase assays using recombinant, GST-tagged CES and CPK3, CPK4, CPK5, CPK6 and CPK11. CBB staining is shown as a loading control. (d) In vitro kinase assays with CPK3 using recombinant, GST-tagged CES mutant variants. CBB staining is shown as a loading control.