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. Author manuscript; available in PMC: 2014 Sep 19.
Published in final edited form as: Virology. 1991 Jul;183(1):366–373. doi: 10.1016/0042-6822(91)90149-6

Fig. 1.

Fig. 1

Outline of construction of plasmids pARgp7, pARgp7-8-8.5, and pARgp7-8-8.5-10 expressing or coexpressing the scaffolding protein gp7, the capsid protein gp8, the fiber protein gp8.5, and the portal protein gp10. Individual DNA fragments were cloned into the Ndel site of the plasmid pET-3c. PT7, bacteriophage T7 promotor; Hairpin, a dyad structure that produces a hairpin loop in RNA which protects the RNA transcripts from degradation by exonuclease; gp, gene product; Term, φ10 transcription termination sequence.