Figure 6.

Skint1 haplotypes in diverse mouse strains. (a) Variants at 40 Skint1 codons, resulting in 35 missense substitutions, 4 silent substitutions and 1 premature termination (found only in FVBTac), are listed next to a schematic of the protein structure, with domains colored as in Figure 3, showing the domain location of the substitutions. The sequences present in B6, FVBJax and FVBTac, as well as 16 other laboratory strains and 5 strains derived from wild mice, are indicated with numbers corresponding to the codons listed at the left. Blue shading within the table highlights B6-like sequence (the reference sequence); Skint1 variation in other strains is shaded yellow (FVB-like), orange (found in I and NON but not FVB), lavender (found only in MOLF), green (found only in CAST) and pink (found only in SPRET). The stop mutation unique to FVBTac is shaded red. Four Skint1 haplotypes are observed among the 16 laboratory strains studied. (b) Presence (+) or absence (−) of an ~540 kb segment of mouse chromosome 4 containing Skint3, Skint4 and Skint9. The segment is present in B6 and 8 other distantly related mouse strains, but absent in FVB and 13 other strains. (c) Skint1 protein schematic, drawn to scale, indicating where missense substitutions occur relative to the protein structure; bar color denotes the strains harboring the substitutions, as shown in a. Missense substitutions are concentrated within the first two TMDs and the intervening loop.