. Author manuscript; available in PMC: 2015 Jan 1.

Published in final edited form as: Vis Neurosci. 2014 Jan;31(1):25–37. doi: 10.1017/S0952523813000515

Table 1.

PCR primers and thermal cycling conditions

Primer pair	Primer sequence	Primer location	Thermal cycling
1	5′TGTCACGGATACTTCCTCTTTGGTC 5′GAGGGCCAACTTTGCTAGAAGAGAC	Opn1sw exon 1 Opn1sw exon 5	94°C for 3 min; 35 cycles of 94°C for 30 sec, 68°C for 1.5 min; 72°C for 10 min
2	5′ttcttccagttctggaatgaatgtttg 5′AGTCCTCAGCAACTGGGAGTAGGAGAAG	Opn1sw intron 2 Opn1sw exon 3	95°C for 9 min; 38 cycles of 94°C for 30 sec, 61.5°C for 40 sec; 72°C for 10 min
3	5′tctcttcttccgtgtagGAATCACAGG 5′acacccttacCTGCTCCAACCAAAG	Opn1mw intron 2/exon 3 Opn1mw intron 3/exon 2	95°C for 9 min; 8 cycles of 94°C for 45 sec, 65°C for 45 sec; 32 cycles of 94°C for 1 min, 65°C for 45 sec; 72°C for 10 min
4	5′GGATCACAGGTCTCTGGTCTC 5′CTGCTCCAACCAAAGATGG	Opn1lw^LlAIS exon 3 Opn1lw^LlAIS exon 3	95°C for 9 min; 8 cycles of 94°C for 45 sec, 59°C for 45 sec; 32 cycles of 94°C for 1 min, 59°C for 45 sec; 72°C for 10 min
5	5′cttctttgccacacttggagGTGAAATC 5′GCCATGATCCAGGTGAAGACCACAC	Rho intron 1/exon 2 Rho exon 2	94°C for 3 min; 35 cycles of 94°C for 30 sec, 68°C for 1.5 min; 72°C for 10 min