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. Author manuscript; available in PMC: 2014 Sep 19.
Published in final edited form as: Hepatology. 2014 Apr 25;59(6):2263–2275. doi: 10.1002/hep.26993

Fig. 5.

Fig. 5

Persistent treatment with LPS induces expression of multiple senescence markers in NHCs. (A) Representative brightfield microscopy images at day 10 demonstrating increased LPS-induced SA-β-gal and cytologic features of senescence (larger size, squamoid appearance). (B) SA-β-gal quantitation reveals significantly increased proportion of SA-β-gal-positive NHCs following 6 and 10 days of LPS treatment. (C) Representative images at day 10 demonstrating increased LPS-induced lysosomal content. (D) Quantitation of lysosomal content reveals a significant increase in lysosomal content at day 6 and a further increase at day 10 compared to control. (E) Representative images at day 10 demonstrating LPS-induced expression of p16INK4a promoter-driven reporter (RFP). (F) Quantitation of p16INK4a (presented as the ratio of RFP to EGFP-positive cells) confirms a significant increase at day 6 and a further increase at day 10 compared to control.