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. Author manuscript; available in PMC: 2014 Sep 19.
Published in final edited form as: Hepatology. 2014 Apr 25;59(6):2263–2275. doi: 10.1002/hep.26993

Fig. 6.

Fig. 6

Persistent LPS treatment of NHCs induces expression of SASP components. (A) IL-6 mRNA expression was assessed by qPCR at 1, 6, and 10 days of LPS treatment. By day 10, expression is significantly elevated (~5-fold > control; ~2-fold > 1 day LPS-treatment). (B) IL-8 mRNA expression is significantly increased by day 10 (~5-fold > control; ~1.5-fold > 1 day LPS-treatment). The delayed response observed in IL-8 (as well as IL-6) expression following an initial acute response is consistent with the time-dependent induction of senescence as demonstrated in other cell types and culture models.33 (C) CCL2 mRNA expression is significantly increased by day 10 (~4.7-fold > control; ~1.9-fold > 1 day LPS-treatment). (D) ELISA of conditioned media from 10-day LPS-treated NHCs demonstrating significantly increased IL-6, IL-8, and CCL2 protein expression compared to control.