Table 1. Optimization process of total RNA extraction.
| Isolation system | Plasma input [ml] | RNA yield [ng] | Sample |
| Assay optimization | |||
| NucleoSpin miRNA Plasma Kit (Macherey-Nagel, Germany) | 0.3 | not quantifiable | |
| NucleoSpin miRNA Plasma Kit (Macherey-Nagel, Germany) | 0.6 | not quantifiable | |
| NucleoSpin miRNA Plasma Kit (Macherey-Nagel, Germany) | 1.0 | not quantifiable | |
| miRNeasy Kit, supplementary control (Qiagen, Germany) | 0.3 | not quantifiable | |
| miRNeasy Kit, supplementary control (Qiagen, Germany) | 0.6 | not quantifiable | |
| miRNeasy Kit, supplementary control (Qiagen, Germany) | 1.0 | not quantifiable | |
| miRNeasy Serum/Plasma Kit (Qiagen, Germany) | 6.0 | 37.2 | |
| miRNeasy Serum/Plasma Kit (Qiagen, Germany) | 6.0 | 61.0 | |
| Analyzed samples | |||
| miRNeasy Serum/Plasma Kit + QIAvac System (Qiagen, Germany) | 9.0 | 57.6 | Animal 1 |
| miRNeasy Serum/Plasma Kit + QIAvac System (Qiagen, Germany) | 9.0 | 36.3 | Animal 2 |
| miRNeasy Serum/Plasma Kit + QIAvac System (Qiagen, Germany) | 9.0 | 72.9 | Animal 3 |
| miRNeasy Serum/Plasma Kit + QIAvac System (Qiagen, Germany) | 9.0 | 38.4 | Animal 4 |
| miRNeasy Serum/Plasma Kit + QIAvac System (Qiagen, Germany) | 9.0 | 45.0 | Animal 5 |
| miRNeasy Serum/Plasma Kit + QIAvac System (Qiagen, Germany) | 9.0 | 51.9 | Animal 6 |
| miRNeasy Serum/Plasma Kit + QIAvac System (Qiagen, Germany) | 9.0 | 112.0 | Animal 7 |
| miRNeasy Serum/Plasma Kit + QIAvac System (Qiagen, Germany) | 9.0 | 71.9 | Animal 8 |
| miRNeasy Serum/Plasma Kit + QIAvac System (Qiagen, Germany) | 9.0 | 210.4 | Animal 9 |
The table compiles the tested plasma input volumes, the used isolation systems and the resulting yields of extracted total RNA [ng] measured with the Qubit 2.0 Fluorometer.