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. Author manuscript; available in PMC: 2015 Oct 1.
Published in final edited form as: Anal Bioanal Chem. 2014 Aug 8;406(25):6203–6211. doi: 10.1007/s00216-014-8081-4

Table 1.

Binding parameters obtained for R- and S-propranolol on a VLDL column at various temperaturesa

Enantiomer & binding model Temperature (°C) mL1 (mol) Ka1 (M−1) nKab (M−1)
R-Propranolol, Two interactions: saturable + non-saturable 20 0.96 (± 0.47) × 10−8 9.2 (± 4.8) × 104 3.0 (± 0.3) × 106
27 1.3 (± 0.8) × 10−8 7.3 (± 4.3) × 104 2.9 (± 0.5) × 106
37 1.3 (± 0.5) × 10−8 7.0 (± 2.3) × 104 1.2 (± 0.3) × 106
S-Propranolol, Two interactions: saturable + non-saturable 20 1.6 (± 0.8) × 10−8 6.9 (± 3.4) × 104 2.5 (± 0.5) × 106
27 2.5 (± 0.9) × 10−8 4.6 (± 1.3) × 104 1.8 (± 0.4) × 106
37 0.78 (± 0.16) × 10−8 9.6 (± 2.2) × 104 2.4 (± 0.6) × 106
a

The numbers in parentheses represent a range of ± 1 S.D. All of these results were measured in pH 7.4, 0.067 M potassium phosphate buffer.

b

The value for nKa for a non-saturable interaction was obtained by dividing the best-fit result for mLKa by the estimated moles of VLDL in the column. This latter value was obtained by using the protein content of the VLDL support, determined using an average molar mass for VLDL of 6.9 × 106 g/mol and a typical protein content for VLDL of 8% (w/w) [8,10,12-14].