Table 1.

List of antibodies with working dilutions for Western Blot and fluorescent immunostaining along with the source used in this study

Antigen	Working dilution	Identity, Source
rat PAM-1:
C-STOP, PAM-1(965–976)	1:1000	C-stop (86)
Exon 16, PAM-1(409–497)	1:1000	JH629 (87)
PHM, PAM-1(37–382)	1:1000	JH1761 (88)
PAL, PAM-1(498–604)	1:1000	JH877 (89)
SG proteins:
PC1(359–373)	1:1000	JH888 (52)
POMC (γ MSH)	1:1000	JH189 (90)
Carboxypeptidase D	1:1000 and see EM	CPD AE142, gift from Dr. Lloyd Fricker (91)
ACTH (Rabbit)	See EM	C-terminus of ACTH (42)
POMC/ACTH (Rabbit)	1:1000	JH93 (N-terminus of ACTH) (92)
AP-1 components:
μ1A	1:500	ProteinTech Group
γ-adaptin	1:1000	BD Biosciences
Golgi:
GM130	1:1000	BD Biosciences
TGN38 (155–249)	1:1000	(39)