Table I.
Ciliary targeting consensus sequences present in cargos
| Cargo | Protein type | Organism | Signal1 (Consensus)a/binding partner | Signal2 (Consensus)a/binding partner |
|---|---|---|---|---|
| ODR-10 | GPCR | C.elegans | FR (ØB)/NDb(170) | |
| STR-1 | GPCR | C.elegans | YR (ØB)/ND (170) | |
| SSTR3 | GPCR | Mammalian | FK (ØB)/BBSome (4) | APSCQ (AX[S,A]XQ)/ND (171) |
| HTR6 | GPCR | Mammalian | FK (ØB)/ND (4) | ATAGQ (AX[S,A]XQ)/ND (171) |
| Rhodopsin | GPCR | Mammalian | FR (ØB)/ASAP1 (4, 46, 170) | QVSPA ([K,R,Q]VXPX)/Arf4 (42, 44) |
| Smoothened | GPCR | Mammalian | WR (ØB)/ND (4) | |
| MCHR1 | GPCR | Mammalian | APASQ (AX[S,A]XQ)/ND (171, 172) | |
| GPR161 | GPCR | Mammalian | [I,V]KARK/ND (113) | |
| Polycystin-1 | GPCR | Mammalian | KVHPSST ([K,R,Q]VXPX)/ND (173) | |
| PGR15L | GPCR | Mammalian | [R,K][I,L]W/ND (142) | |
| GPR83 | GPCR | Mammalian | [R,K][I,L]W/ND (142) | |
| NPY2R | GPCR | Mammalian | [R,K][I,L]W/ND (142) | |
| Polycystin-2 | 6 TM domains | Mammalian | RVQPQ ([K,R,Q]VXPX)/ND (49) | |
| Fibrocystin | Single-pass TM | Mammalian | CLVCCWFKKSKTRKIKP/Rab8 (174) | |
| RP2 | Membrane-associated protein | Mammalian | Consensus M9 sequence/Importin β2 (99) | |
| CNGB1b | Ion channel | Mammalian | RVSPG ([K,R,Q]VXPX)/ND (175) | |
| KIF17 | Soluble | Mammalian | KRKK (Similar to NLS)/Importinβ2 (98) |
a
Amino acids are indicated using the 1-letter code (e.g., Y=Tyrosine; N=Asparagine). “X” represents a position occupied by any amino acid. Ø= amino acid with a bulky-hydrophobic side chain (L, I, M, V, F). Amino acids within brackets indicate that one or the other can be found in that position within the consensus.
b
Not determined