Table 1.

Sample of cross-sectioned synapses.

Condition	Description	PSD area (μm2)	AZ area (μm2)	NZ area (μm2)	# NZ/PSD
Perfusion-fixed	N	124	124	48	43
	mean ± SD	0.054 ± 0.037	0.050 ± 0.035	0.011 ± 0.009	1.1 ± 0.4
	range	0.013 – 0.169	0.009 – 0.169	0.005 – 0.051	3
5 min control	N	189	189	95	71
	mean ± SD	0.073 ± 0.060	0.069 ± 0.055	0.009 ± 0.005	1.3 ± 0.8
	range	0.007 – 0.366	0.007 – 0.353	0.004 – 0.032	4
5 min LTP	N	146	145	72	61
	mean ± SD	0.076 ± 0.073	0.071 ± 0.067	0.010 ± 0.009	1.2 ± 0.6
	range	0.007 – 0.609	0.007 – 0.540	0.004 – 0.052	4
30 min control	N	201	201	161	113
	mean ± SD	0.072 ± 0.076	0.063 ± 0.067	0.011 ± 0.009	1.4 ± 0.8
	range	0.006 – 0.610	0.002 – 0.525	0.004 – 0.058	5
30 min LTP	N	236	236	168	135
	mean ± SD	0.070 ± 0.062	0.063 ± 0.058	0.010 ± 0.008	1.2 ± 0.6*
	range	0.011 – 0.544	0.004 – 0.490	0.004 – 0.056	4
2 hr control	N	153	152	92	79
	mean ± SD	0.057 ± 0.053	0.053 ± 0.050	0.008 ± 0.005	1.2 ± 0.4
	range	0.006 – 0.364	0.006 – 0.318	0.004 – 0.039	3
2 hr LTP	N	246	245	153	121
	mean ± SD	0.089 ± 0.089****	0.080 ± 0.081****	0.015 ± 0.012****	1.3 ± 0.6
	range	0.006 – 0.661	0.006 – 0.572	0.005 – 0.082	5

Includes: number of synapses (N), mean ± standard deviation (SD), and ranges for PSD, AZ, and NZ areas, and the maximum number of NZs per synapse for synapses that had NZs (#NZ/PSD) for perfusion-fixed tissue and control and LTP slices at increasing experimental time points. Statistical comparisons of LTP versus time-locked controls for AZ areas (hnANOVAs, 5 min, F_(1,314) = 0.63, p = 0.43; 30 min, F_(1,412) = 1.2, p = 0.27; 2 hr, F_(1,379) = 22.1, p < 0.0001****). Statistical comparisons of LTP versus time-locked controls for individual NZ areas (hnANOVAs, 5 min, F_(1,147) = 0.54, p = 0.46; 30 min, F_(1,304) = 2.0, p = 0.16; 2 hr, F_(1,227) = 42.1, p < 0.0001****) and for the number of NZs per PSD (hnANOVAs, 5 min, F_(1,112) = 3.3, p = 0.071; 30 min, F_(1,223) = 4.6, p = 0.032*; and 2 hr, F_(1,182) = 3.0, p = 0.084).