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. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: J Comp Neurol. 2014 Aug 25;522(17):3885–3899. doi: 10.1002/cne.23647

Figure 4. Total TrkB and BDNF levels in whole hippocampal homogenates comparing ZnT3−/− and ZnT3+/+ animals.

Figure 4

A) Representative Western blot demonstrating TrkB levels in ZnT3+/+ and ZnT3−/− mice assayed in whole hippocampal lysates. FL TrkB = full length TrkB. Trunc. TrkB = truncated TrkB. B) Quantification of full-length TrkB levels demonstrates no significant difference between wild type (n=13) and knockout (n=11) animals. Data are represented as mean percent of average TrkB levels found in the ZnT3+/+ animal ± SEM, and was normalized to β-actin as a loading control. C) Total BDNF levels in whole hippocampal lysates assayed by ELISA demonstrates an approximately 52% increase in the ZnT3−/− (n=10) compared to the ZnT3+/+ (n=8) mouse. Data are represented as ng BDNF levels per gram of total protein ± SEM. All data were analyzed by Student’s t-test; **p<0.01.