TABLE 5.
Sample | Mutation | CD34+/CD33− Cells | CD34+/CD33+ Cells |
---|---|---|---|
Short-term culture (2 weeks) | |||
1. 798451 (BM) | inv(16) + KIT | No mutation | ---* |
2. 781469 (BM) | t(8;21) + KIT | --- | CBF+ only |
3. 26450 (PB) | inv(16) + NRAS | CBF+ only | ---* |
4. 001-001-012 (PB) | inv(16) + FLT3/ITD | CBF+ only | CBF+ only as well as both mutations |
5. 791406 (BM) | t(8;21) + FLT3/ITD | Both mutations | Both mutations |
6. 792590 (BM) | t(8;21) + KIT | Both mutations | Both mutations |
7. 774353 (BM) | t(8;21) + KIT | Both mutations | Both mutations |
Long-term culture (4–8 weeks) | |||
1. RO50397 (BM) | t(8;21) + KIT | No mutation | No mutation** |
2. 766411 (BM) | inv(16) + KIT | --- | CBF+ only |
3. 774353 (BM) | t(8;21) + KIT | No mutation | Both mutations |
4. 792590 (BM) | t(8;21) + KIT | No mutation | CBF+ only as well as both mutations |
5. 26450 (PB) | inv(16) + NRAS | CBF+ only | --- |
6. 775184 (BM) | inv(16) + KIT | CBF+ only | --- |
CBF+ only CFC-GMs were isolated from short-term cultured unsorted cells.
CBF+ only CFC-GMs were isolated from long-term cultured unsorted cells.
Abbreviations: “---“ denotes no CFU-GM growth; BM, bone marrow; PB, peripheral blood.