TABLE 5.

Growth and molecular analysis of CFU-GMs derived from short-term (2 weeks) or long-term (4–8 weeks) culture of primary hematopoietic cells from patients with CBF leukemias and complementing second mutation

Sample	Mutation	CD34+/CD33− Cells	CD34+/CD33+ Cells
Short-term culture (2 weeks)
1. 798451 (BM)	inv(16) + KIT	No mutation	---*
2. 781469 (BM)	t(8;21) + KIT	---	CBF+ only
3. 26450 (PB)	inv(16) + NRAS	CBF+ only	---*
4. 001-001-012 (PB)	inv(16) + FLT3/ITD	CBF+ only	CBF+ only as well as both mutations
5. 791406 (BM)	t(8;21) + FLT3/ITD	Both mutations	Both mutations
6. 792590 (BM)	t(8;21) + KIT	Both mutations	Both mutations
7. 774353 (BM)	t(8;21) + KIT	Both mutations	Both mutations
Long-term culture (4–8 weeks)
1. RO50397 (BM)	t(8;21) + KIT	No mutation	No mutation**
2. 766411 (BM)	inv(16) + KIT	---	CBF+ only
3. 774353 (BM)	t(8;21) + KIT	No mutation	Both mutations
4. 792590 (BM)	t(8;21) + KIT	No mutation	CBF+ only as well as both mutations
5. 26450 (PB)	inv(16) + NRAS	CBF+ only	---
6. 775184 (BM)	inv(16) + KIT	CBF+ only	---

^*CBF+ only CFC-GMs were isolated from short-term cultured unsorted cells.

^**CBF+ only CFC-GMs were isolated from long-term cultured unsorted cells.

Abbreviations: “---“ denotes no CFU-GM growth; BM, bone marrow; PB, peripheral blood.