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. 2014 Sep 13;7:643. doi: 10.1186/1756-0500-7-643

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© Oh et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Tissue specific cosuppression of HSP90.5 in transgenic lines. Immunoblotting of total soluble proteins (7 μg) from different tissues were analyzed. Anti-FLAG, anti-HSP90.5 or anti-HSP90.2 antibody is used. Plants grown for 3 weeks were analyzed. Wild type (WT), normally growing T3 transgenic lines no. 3 (3G), no. 57 (57G), and variegated transgenic plants no. 3 (3A), no. 57 (57A) and no. 8 (8A) were analyzed. A and B, Examination of different rosette leaves. C, Analysis of different regions of a variegated leaf. The 6^th rosette leaf that developed partially albino phenotype was sectioned along the yellowish transition line to separate the albino proximal (P) region from the still green distal (D) region. D, Examination of total soluble proteins extracted from unopened flower buds (B), inflorescence stem (S), the 6^th leaf (L) and roots (R).