Table 1.
Name | Primer sequence | PCR conditions | Size (bp) |
CCK-BR | 1: 5’TCTCGCGAGCTCTACTTAGGG3’ | 94 °C, 30 s | 185 |
2: 5’ACCGACGATGCACGTTGAAG3’ | 62 °C, 30 s | ||
72 °C, 30 s | |||
ERK1/2 | 1: 5’TATTCCCGGGCAAGCACTATTT3’ | 94 °C, 30 s | 243 |
2: 5’CGGGCTCATCATTCGGGTCGTA3’ | 54 °C, 30 s | ||
72 °C, 30 s | |||
K-ras | 1: 5’ACAGTGCAATGAGGGACCAGTA3’ | 94 °C, 30 s | 275 |
2: 5’GTATAGAAGGCATCATCAACACC3’ | 50 °C, 30 s | ||
72 °C, 30 s | |||
Actin | 1: 5’ATGATATCGCCGCGCTCGTCGTC3’ | 94 °C, 30 s | 342 |
2: 5’CGCGGTTGGCCTTGGGGTTCAG3’ | 60 °C, 30 s | ||
72 °C, 30 s |
PCR: Polymerase chain reaction; CCK-BR: Cholecystokinin-B receptor; ERK1/2: Extracellular signal-regulated protein kinase 1/2.