Skip to main content
. 2014 Sep 21;20(35):12542–12550. doi: 10.3748/wjg.v20.i35.12542

Table 1.

Primers used for nested reverse transcriptase polymerase chain reaction amplification of cholecystokinin-B receptor, extracellular signal-regulated protein kinase 1/2 and K-ras

Name Primer sequence PCR conditions Size (bp)
CCK-BR 1: 5’TCTCGCGAGCTCTACTTAGGG3’ 94 °C, 30 s 185
2: 5’ACCGACGATGCACGTTGAAG3’ 62 °C, 30 s
72 °C, 30 s
ERK1/2 1: 5’TATTCCCGGGCAAGCACTATTT3’ 94 °C, 30 s 243
2: 5’CGGGCTCATCATTCGGGTCGTA3’ 54 °C, 30 s
72 °C, 30 s
K-ras 1: 5’ACAGTGCAATGAGGGACCAGTA3’ 94 °C, 30 s 275
2: 5’GTATAGAAGGCATCATCAACACC3’ 50 °C, 30 s
72 °C, 30 s
Actin 1: 5’ATGATATCGCCGCGCTCGTCGTC3’ 94 °C, 30 s 342
2: 5’CGCGGTTGGCCTTGGGGTTCAG3’ 60 °C, 30 s
72 °C, 30 s

PCR: Polymerase chain reaction; CCK-BR: Cholecystokinin-B receptor; ERK1/2: Extracellular signal-regulated protein kinase 1/2.