Figure 3.
p41 induces murine Tr1/Treg in vivo depending on adjuvant. Immunofluorescence staining of lungs (a) and flow cytometry of lungs (b) and TLN (c) from C57BL/6 mice intranasally treated with anti-CD3, the p41-peptide or the control C22 peptide along with zymosan or CpG-ODN. Lungs were stained with anti-LAP-PE, anti-IL-10-PE or anti-Foxp3 followed by PE secondary antibody and 4',6-diamidino-2-phenylindole for nuclei for Tr1/Treg-cell assessment at the end of each treatment. Representative images of two independent experiments were acquired with a × 40 objective. Scale bars, 200 μm. Total cells were stimulated in vitro with anti-CD3+CD28 for 24 h before surface staining with anti-CD4-FITC (fluorescein), anti-CD25-PE and anti-LAP-PE. Numbers refer to % positive cells.