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. 2014 May 13;92(8):659–670. doi: 10.1038/icb.2014.34

Figure 6.

Figure 6

Mouse Tr1 cells are activated through Ahr and require IL-10 and, partly, TGF-β to suppress. Ahr−/− or Il10−/− mice were treated and infected as decribed previously in Figure 5 and assessed for lung immunofluorescence with anti-LAP-PE or anti-IL-10-PE staining (a, c); numbers of LAP+ CD4+ T cells by flow cytometry on anti-CD3+CD28-stimulated lung cells (b, d), and lung histology (periodic acid-Schiff staining) (e, f). Cell nuclei were stained blue with 4′,6-diamidino-2-phenylindole in a and c. Representative images of two independent experiments were acquired with a × 40 objective. Scale bars, 200 μm. In b and d, numbers refer to % positive cells. (g) C57BL/6 mice were treated with p41+zymosan, infected and treated with IL-10 or TGF-β-neutralizing antibody at the time of infection. Scale bars, 200 μm. None, rat IgG1 antibody.