Fig. 3.

Effect of KYNA on β-catenin expression and cellular localization in HT-29 cells. Western blot analysis of protein expression of β-catenin in HT-29 cells after treatment with KYNA 1 mM for 5 min–48 h (a) or treatment with KYNA in the range of concentrations 0.01–5 mM for 24 and 48 h (b); (C control; not treated). Western blots shown in the figure were selected as the most representative of the series of repetitions n ≥ 3. The data were normalized relative to β-actin. The results of densitometric analysis are shown as % of control (the changes ≥20 % were considered as significant). c Immunofluorescent staining for β-catenin in HT-29 cells treated with KYNA 5 mM for 24 h (control; not treated). Magnification×400