Figure 6. Additive effect of rhEndostatin with CTX on the metastasis of B16 melanoma.

Nude mice (n = 10, each) were inoculated with 2.5×10⁵ tumor cells intravenously to establish a metastasis model. Mice were either administered i.v. with rhEndostatin alone at 10 mg/kg/day, 5 mg/kg/day, or 2.5 mg/kg/day for 15 days successively, or combined treated with CTX at the dosage of 15 mg/kg/d for successively 7 days, then intravenously administered with rhEndostatin at 10 mg/kg/day, 5 mg/kg/day, or 2.5 mg/kg/day for successively 15 days. The mice were sacrificed to count the metastasis colony in the lung on day 25 post inoculation. The formula, [(mean colony of PBS control- mean colony of treated mice)/mean colony of PBS control]×100, was used to calculate the inhibition rate of metastasis. #: P<0.05 vs mice administered with single rhEndostatin treatment at the same dose, respectively; *: P<0.05 vs mice treated with CTX alone, at the dosage of 15 mg/kg/day.