Figure 4.

Nucleotide-binding oligomerization domain 2 (NOD2) enhances Campylobacter jejuni clearance in macrophages. Elicited peritoneal macrophages from Il10^−/− and Il10^−/−; Nod2^−/− mice were infected with C. jejuni, and bacterial survival was determined using a gentamicin assay. A, C. jejuni invasion in macrophages at 0 and 2 hours was enumerated by plating. B, Representative images of C. jejuni (red dots) invasion into macrophages, using 5-Cyano-2,3-di-(p-tolyl) tetrazolium chloride-labelled C. jejuni. Scale bar is 10 μm. Data represent means ± standard errors of the mean. Results are representative of 3 independent experiments. *P < .05. Abbreviations: CFU, colony-forming units; NS, not significant.