Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Sep 19;8:285. doi: 10.3389/fncel.2014.00285

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2014 Thériault and Chahine.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

mRNAs reflect electrophysiological properties. (A) Representative current-traces recorded from small diameter DRG neurons (28 μm in diameter) before (right) and after (left) the addition of 1 μM TTX. The protocol is shown in the inset. (B) Example of current-voltage (I–V) relationships obtained from the small diameter DRG neuron shown in (A). The dark red trace is the total current recorded under control conditions, the orange trace is the total TTX-R current recorded in presence of 1 μM TTX, and the green trace is the TTX-S current obtained by subtracting the TTX-R Na_v current (orange) from the total Na_v current (dark red). I–V are fitted using a Hodgkin–Huxley-like equation: f = G_max*(V − V_rev)/ (1 + exp((V − V_1/2)/k)), where G_max is the maximal conductance, V the potential, V_1/2 is the voltage at which half of the channels are in the open state, V_rev is the reversal potential, and k is the slope factor. (C) Activation curves obtained from (B) illustrating the conductance of total Na_v current (dark red), TTX-R current (orange) and TTX-S current (green). The conductance was calculated using the following equation: G_Na = I_Na/(V_m − V_rev), where G_Na is the conductance, I_Na is the peak current for the test potential V_m, and V_rev is the reversal potential estimated from the current-voltage curve. (D) Correlation of normalized TTX-R Na_v currents to normalized mRNA coding for TTX-R channels (Na_v1.8 + Na_v1.9/(Na_v1.7 + Na_v1.8 + Na_v1.9)). Currents were normalized to the maximal current. n = 17 from 8 animals.