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DNA denaturation by the chemical treatments. The treatments include (A) 1 mol/L NaOH (closed circles), 0.1 mol/L NaOH (open circles), or 0.01 mol/L NaOH (closed triangle), (B) 25% formamide (closed circles) or 25% formamide (open circles), and (C) 60% DMSO (closed circle), 50% DMSO (open circles), or 25% DMSO (closed triangle). DMSO, dimethyl sulfoxide.
