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. 2014 Sep 9;2:e555. doi: 10.7717/peerj.555

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© 2014 Hao et al.

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.

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(A) Fluorescent staining of nuclei in A549 cells by Hoechst 33342. (B) Flow cytometry assay to detect apoptosis in A549 cells using Annexin V staining. There is no significant difference among control group and psoralidin treated groups. NS, no significant vs. Con. (C) The expression of apoptosis related proteins were analyzed by Western blot in A549 cells receiving psoralidin treatment. (D) The cell viability was measured by MTT assay after treatment with psoralidin in the absence or presence of Z-VAD-FMK or Ac-DEVD-CHO. ^∗∗P < 0.01 vs. Con; NS, no significant vs. psoralidin only-treated group. Con, control.