Figure 4. Psoralidin induced autophagic cell death in A549 cells.

(A) Cells were stained with MDC after psoralidin treatment. (B) Immunofluerescence analysis of endogenous LC3 in psoralidin-treated A549 cells. (C) Cells were treated with indicated concentrations of psoralidin for 24 h. The expression of LC3 was determined by Western blot. (D) Cells were treated with 20 µM psoralidin for 6, 12, 24 or 48 h, expression of LC3 was determined by Western blot. (E) Cells were treated with psoralidin, 3-MA or a combination of both, expression of LC3 was analyzed by Western blot. (F) The cell viability was measured by MTT assay after treatment with psoralidin in the absence or presence of 3-MA. ^∗∗P < 0.01 vs. Con, ^#P < 0.05 vs. psoralidin only-treated group. Con, control.