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Kinetic characterization of the sequence-specific Cu-free Sonogashira cross-coupling. Time courses of the cross-coupling reaction between the preactivated fluorescein iodide–Pd complex and the fusion protein Ub-G4 (a) or Ub-R1–4 (b) as monitored by LC-MS. 2.5 μM of the fusion protein was incubated with 20 μM of the preactivated fluorescein iodide–palladium complex in PBS at 37 °C. SM = starting material; Prod = cross-coupling product.
