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Fluorescence quenching assays of YxeB-L142-6×His (panel A) and YxeB-S142-6×His (panel B). The dissociation constants were calculated by Hyperquad22 using the assay data (see Table 1). Open squares, AcDFO; closed squares, AcFO; closed triangle, Ga-DFO; open triangles, TBS buffer (control). The fluorescence quenching curves of YxeB-L142-6×His for AcFO and Ga-DFO and of YxeB-S142-6×His for AcFO, AcDFO, and Ga-DFO were fit to the calculated quenching curves (lines) by Hyperquad.22
