Figure 3.

Activity of subgroup of GH1s toward phosphorylated substrates. (a) Multiple sequence alignment highlighting the SKY/SNY motif found in a subgroup of GH1s that showed no activity toward the NIMS substrates, along with the sequence from a 6-phospho-β-glucosidase GH1 from L. lactis with a known crystal structure (top). Four representative GH1s from this study that showed activity toward the NIMS and real substrates are shown for comparison (bottom). (b) A phylogenetic tree highlighting the subgroup of GH1s with the SKY/SNY motif; the three outer concentric rings show (i) expression, (ii) SKY/SNY motif identity, and (iii) their enzyme activity toward the phosphorylated chromogenic substrate, pNPβG6P.