Figure 3.

Analysis of Wnt3 and Wnt9b expression in mid-gestational mouse embryos by in situ hybridization. (A) Expression of Wnt3 was widely detected from E9.5 to E15.5, with stronger expression observed in the skin epithelia, limb buds and neural tissues. Wnt3 is also present in the genital tubercle at these stages. Expression at E14.5 and E15.5 is not shown. gt, genital tubercle; tl, tail. (B) Expression was detectable between E9.5 and E13.5 in the epithelia of the branchial arches, frontonasal process, limb buds and genital tubercle (including the cloacal membrane between E9.5 and E10.5). Strong expression was also found in the nephric ducts. The expression pattern of E13.5 (not shown) was identical to that at E12.5. ba, branchial arch; cm, cloacal membrane; flb, forelimb bud; fnp, frontonasal process; gt, genital tubercle; hlb, hindlimb bud; nd, nephric duct; nt, neural tube; tl, tail.