Figure 3.

Impact of internalized SPIOs on gene expression. Wild type BALB/c mice were intravenously injected with PBS (iron free control), Ferinject^® (4.6 mg iron per mouse); polymer-coated SPIOs (100 µg iron per mouse) and SPIOs-micelles (50 µg iron per mouse). Gene expression analysis was performed for (A) TNFα, (B) Il1b and (C) Ccl2. The injection of high iron doses by using Ferinject^® but not the injection of SPIOs increased the expression of pro-inflammatory TNFα and Ccl2. Mean values +/− s.d. with n ≥ 4, *p < 0.05