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. 2014 Aug 27;5:1380–1392. doi: 10.3762/bjnano.5.151

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Copyright © 2014, Docter et al.

This is an Open Access article under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

The license is subject to the Beilstein Journal of Nanotechnology terms and conditions: (https://www.beilstein-journals.org/bjnano/terms)

PMC Copyright notice

Automated microscopy to analyze the impact of exposure to ASP30 on the cell viability. Caco-2 cells were exposed in DMEM to the indicated ASP30 concentrations for 4 h in the absence (w/o protein; left panel) or presence (+-proteins; right panel) of 10% FCS, washed with buffer A, and the vitality was evaluated by using the two-color fluorescence assay. Intensity of calcein (living cells; green) and ethidium homodimer-1 (dead cells; red) signal was monitored using the Cellomics ArrayScan^® VTI. A minimum of 500 cells were analyzed per well, and each treatment was done in triplicate. As a positive control, cells were treated with methanol (+ control), resulting in 100 % damaged cells.