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. 2014 Aug 27;5:1380–1392. doi: 10.3762/bjnano.5.151

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Copyright © 2014, Docter et al.

This is an Open Access article under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

The license is subject to the Beilstein Journal of Nanotechnology terms and conditions: (https://www.beilstein-journals.org/bjnano/terms)

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Uptake of fluorescently labeled ASPs. (A/B) Automated microscopy to quantify time-dependent cellular uptake of fluorescent amorphous silica nanoparticles (ASP30F (A) and ASP30F-COOH (B)). CaCo-2 cells were exposed to the nanoparticles (100 µg/mL) suspended in DMEM (filled rectangles) or DMEM containing 10% human plasma (open rectangles) for 60 min. Total cellular fluorescence is displayed as arbitrary units (AU). Assays were performed in triplicates. Columns, mean; bars, ± SD from three independent experiments.