Figure 3. Detection of role for SUMO pathway components in epigenetic gene silencing. (A) A schematic representation of the SUMO modification pathway. (B) Reactivation of GFP reporter genes after knockdown of the indicated SUMO pathway components. The reporter cells were transfected with the indicated siRNAs targeting the SUMO pathway (four individual siRNAs per tested gene), and the percent of GFP-positive cells was measured 96 h post transfection. SiRNA pools targeting GAPDH and HDAC1 were used as quadruplicate negative and positive controls, respectively. Error bars indicate standard deviation, n = 6. (C) A time course of GFP reporter gene reactivation after siRNA knockdown of indicated genes. siControl, negative control siRNA.