Figure 4. Knockdown of factors identified in the genome-wide screen results in reactivation of epigenetically silent cellular genes. (A) GFP-silent reporter cells were transfected with selected siRNAs. Levels of the indicated cellular mRNAs were measured 72 h post siRNA transfection by real-time PCR. The heat map shows the fold-increase relative to siControl-transfected cells, P < 0.05. (B) RNA levels of selected cellular genes after transfection with indicated siRNAs. Error bars indicate standard deviation, n = 3, with *p-value < 0.05 and **p-value < 0.01.