Figure 2. Loss of ITIH5 protein expression in human colon cancer. (A–C) Strong ITIH5 expression in epithelial cells of normal colon tissue, especially in goblet cells (arrows in A+B). Clear staining in stroma-associated cells (arrow in c). (D, E) Moderate and low ITIH5 immunoreactivity in cells of colon carcinoma. (F, G) Very low staining in progressed colon cancer cells. (H) Strong ITIH5 protein expression in FFPE section of placenta tissue that served as positive control for ITIH5 staining. (I) Negative control of normal colon tissue. The application of primary antibody was omitted. Scale bar: 50 µm.