Figure 4. DSC thermal stability profiles. (A) Wild-type IgG4 antibody, (B) monovalent half-antibody and (C) monovalent aglycosylated half-antibody. The initial peak is unfolding of the CH2 domain followed by a second unfolding event for all other domains. The CH2 domain of the monovalent half-antibody has an approximate 9°C decrease in stability with a further 5°C decrease for the aglycosylated format.