Table 1. A summary of the IgG4 Fc mutants analyzed by SEC.
| IgG4Fc Mutant | Retention time (min) | Calibrated weight (kDa) | MALLS weight (kDa) | |
|---|---|---|---|---|
| Dimer | E356RK392DR409D | 19.6 | 59.0 | |
| T366W | 19.7 | 59.5 | 53 | |
| T366D | 20.3 | 54.0 | ||
| K439D | 20.5 | 52.5 | ||
| K370W | 20.5 | 52.5 | ||
| K392AK439A | 20.5 | 52.5 | ||
| K439A | 20.6 | 51.5 | ||
| WT | 20.6 | 51.5 | 52 | |
| R409A | 20.6 | 51.5 | ||
| D399W | 20.7 | 51.0 | ||
| S364W | 20.7 | 51.0 | ||
| S354D | 20.7 | 51.0 | ||
| K370A | 20.7 | 51.0 | ||
| E356AK392A | 20.7 | 51.0 | ||
| K392D | 20.8 | 50.0 | ||
| E356A | 20.8 | 50.0 | ||
| E356R | 20.8 | 50.0 | ||
| R409D | 20.9 | 49.0 | ||
| D399A | 21.0 | 48.0 | ||
| S354W | 21.0 | 48.0 | ||
| Monomer-dimer equilibrium | T366DY407D | 20.7 | 51.0 | |
| D399WR409W | 21.0 | 48.0 | ||
| D399AK439A | 21.1 | 47.5 | ||
| T366QY407Q | 21.1 | 47.5 | ||
| F405A | 21.1 | 47.5 | 50 | |
| E356RR409D | 21.1 | 47.5 | ||
| L351W | 21.1 | 47.5 | ||
| K392DK439D | 21.2 | 46.5 | ||
| Y349D | 21.4 | 44.5 | ||
| L368W | 21.5 | 44.0 | ||
| Y407Q | 21.6 | 43.5 | ||
| T366Q | 21.7 | 42.0 | ||
| E356RK392D | 21.8 | 41.5 | ||
| E356AD399A | 22.0 | 40.0 | ||
| T394W | 22.0 | 40.0 | ||
| Y407A | 22.1 | 39.5 | ||
| L351WT394W | 22.2 | 38.5 | ||
| Monomer | Y407D | 22.0 | 40.0 | |
| T394R | 22.1 | 39.5 | ||
| T366R | 22.3 | 37.5 | 35 | |
| R409W | 22.3 | 37.5 | ||
| E357W | 22.4 | 37.0 | ||
| Y407R | 22.4 | 37.0 | 32 | |
| D399R | 22.5 | 36.5 | ||
| T366RY407R | 22.5 | 36.5 | 32 | |
| F405AY407A | 22.6 | 36.0 | ||
| T366QF405QY407Q | 22.7 | 35.0 | ||
| T394D | 22.8 | 34.0 | 28 | |
| F405Q | 22.9 | 33.5 | ||
| S364R | 22.9 | 33.5 | ||
| L351DT394D | 23.0 | 33.0 | ||
| L368R | 23.0 | 33.0 | ||
| L351D | 23.0 | 33.0 | 29 | |
| S364RL368R | 23.1 | 32.0 | ||
| L351R | 23.1 | 32.0 | 30 | |
| F405R | 23.1 | 32.0 | 29 | |
| L351RT394R | 23.2 | 31.5 | ||
| S364WL368W | 23.3 | 31.0 | ||
| E357R | 23.4 | 30.0 | ||
| D399RK439D | 23.4 | 30.0 | ||
| E356RD399R | 23.4 | 30.0 | ||
| T366WL368W | 23.7 | 28.0 | ||
| L351RS364RT394R | 25.1 | 26.0 |
The samples are split into three groups and ordered by retention time with calibration of the column used to estimate molecular weight. The calculated molecular weight from SEC-MALLS is also shown for those samples for which data was collected.