Figure 3. Ability to stimulate internalization does not correlate with ELISA score. (A) Internalization efficiency does not correlate with ELISA score. A scatter plot of internalization efficiency vs. ELISA score rank (listed in the order from higher to lower ELISA scores) for a total of 18 frequently occurring internalized anti-p75^NTR clones showed no significant correlation. (B) In vitro panning increased the relative proportion of frequently occurring phage clones displaying anti-p75^NTR antibodies with ELISA scores above 1.5 (blue columns), whereas internalized clones with lower ELISA scores were lost through selections (red columns). (C) Internalizing anti-p75^NTR antibodies are lost with increasing rounds of in vitro panning. The proportion of the phage population that internalized (red columns) decreased as the mean ELISA scores of the phage population increased with repeated rounds of in vitro panning (blue columns).