Table 2. Tested component concentration ranges.
| Components | Level 0 (mM) | Level 2 (mM) |
|---|---|---|
| NaH2PO4 | 1.7 | 10 |
| L-Leucine | 2 | 9 |
| L-Lysine | 1 | 6 |
| Glycine | 0 | 3 |
| L-Methionine | 0.4 | 2 |
| L-Glutamic acid | 1 | 4 |
| L-Phenylalanine | 0.5 | 3 |
| L-Proline | 0.7 | 6 |
| L-Threonine | 0.7 | 6 |
| L-Tryptophan | 0.5 | 2 |
| L-Valine | 1 | 7 |
| Magnesium Sulfate | 0.1 | 1.5 |
| Calcium Chloride | 0.1 | 1.05 |
| myo-Inositol | 0.07 | 0.7 |
| Sodium pyruvate | 0.8 | 4 |
| D-Biotin | 0.0008 | 0.01 |
| Choline Chloride | 0.1 | 1 |
| L-Aspargine | 3 | 9 |
| Folic acid | 0.006 | 0.04 |
| Niacinamide (B3) | 0.03 | 0.15 |
| D-Pantothenic acid x 1/2Ca | 0.015 | 0.15 |
| L-Serine | 1 | 8 |
| Potassium Chloride | 1 | 10 |
| Pyridoxine | 0.005 | 0.05 |
| L-Aspartic acid | 0.8 | 2.4 |
| Riboflavin | 0.0003 | 0.003 |
| Thiamine | 0.008 | 0.04 |
| Ferric ammonium citrate (mg/L) | 1 mg/L | 10 mg/L |
| Vitamin B12 | 0.0003 | 0.004 |
| Hypoxanthine | 0.008 | 0.04 |
| Thymidine | 0.0015 | 0.006 |
| Putrescine | 0.006 | 0.03 |
| Ethanolamine | 0.1 | 0.5 |
| Zinc Sulfate | 0.004 | 0.02 |
| Cupric sulfate | 0.00004 | 0.0008 |
| Pluronic (g/L) | 0.5 g/L | 2.0 g/L |
| L-Tyrosine | 0.7 | 3 |
| Sodium Selenite | 0.00001 | 0.00006 |
| L-Alanine | 0 | 3 |
| L-Arginine | 1 | 3 |
| L-Cysteine | 1 | 3 |
| L-Histidine | 0.4 | 3 |
| L-Isoleucine | 1 | 6 |
43 among 47 medium components were tested at three levels, level 0 (low), level 1 (intermediate) and level 2 (high). Level 1 is not shown but represents an intermediate concentration between level 0 and level 2 for each component. Glucose (6g/L) and NaHCO3 (2g/L) were kept constant, while NaCl and NaOH were used for osmolality and pH adjustments.