Table 1.

PCR and pyrosequencing primer sequences

Genes	Analyzed zones and primer sequences
fasn
−90 to −62	Fwd Rev Seq	5′-GTGTGGAAGTTAGATGATAATT-3′ 5′-BIO-CTTAAAACTCTAATCTTATAACCACCTTAC-3′ 5′-GTTAGATGATAATTTTTAAGTGGG-3′
+754 to +812	Fwd Rev Seq	5′-GGTTTGGGATTGGAAAGAGATT-3′ 5′-BIO-ACATATCTAAACTATATCCTTTTTACACCA-3′ 5′-GGATTGGAAAGAGATTGA-3′
+951 to +997	Fwd Rev Seq	5′-GAATTTTGGAAAATAAATATAGTGAGTGT-3′ 5′-BIO-ATAACCCTTAATCCCCAACACT-3′ 5′-ATTTTGGAAAATAAATATAGTGAG-3′
pnpla2
−205 to −162	Fwd Rev Seq	5′-GTAGGGTGTGGTGGAGAT-3′ 5′-BIO-TTCTACCCCTCCTACTACACT-3′ 5′-GGGTGTGGTGGAGAT-3′
+150 to +172	Fwd Rev Seq	5′-BIO-GGTGTTTAGATTTGTATAAAATTTG-3′ 5′-CACCTACTAAACAAAACCATCT-3′ 5′-TCTCAAACTTTAACTTCTCAT-3′
+349 to +391	Fwd Rev Seq	5′-GATGTTTTTAAGGGAGATTAAGT-3′ 5′-BIO-AACCACTCCAATATAATAAACC-3′ 5′-GATTAAGTGGAATATTT-3′
pparg
−431 to −388	Fwd Rev Seq	5′-GGTTAGGAGGGTTATAGTGGAGTT-3′ 5′-BIO-AACTATCACCAAATCCACACAAT-3′ 5′-GGTTTTTTTAGAAGGTGTT-3′
−214 to −190	Fwd Rev Seq	5′-BIO-GGTGATAGTTTAAGTAATTTGGT-3′ 5′-ATAACCCCATTTTCCTCA-3′ 5′-CATTTTCCTCACACCTA-3

Fwd forward, Rev reverse, Seq sequence, BIO biotinylated, fasn fatty acid synthase, pnpla2 adipose tissue triglyceride lipase, pparg peroxisome proliferator-activated receptor γ