Figure 6. Over-expression of mutant γ2 subunits decreased surface levels of partnering subunits.
A. B. Wildtype or mutant γ2LHA (A) or γ2SHA (B) subunits were coexpressed with α1β2 subunits in HEK293T cells. Surface and total levels of α1 or β2 subunits were evaluated using flow cytometry. The mock-subtracted mean fluorescence value of each subunit under different experimental conditions were normalized to those obtained with cotransfection of wildtype α1β2γ2LHA (A) (n ≥ 9, mean ± SEM) or α1β2γ2SHA (B) (n ≥ 5, mean ± SEM) subunits. Differences compared to wildtype α1β2γ2 receptor condition were analyzed by the one way ANOVA test followed by Dunnett's multiple comparison test. (*** p < 0.001; ** p < 0.01; * p < 0.05).