Table 3.

Analysis of T cell responses performed by IFN-γ ELISpot after initial injection (bold) and after readministration. The time points for study are described in table S1. Most of the samples tested for T cell responses to the AAV capsid or the RPE65 transgene product were negative throughout the initial (18) and FO studies. A few samples tested positive in the assay (for example, CH12, FO week 6); however, these samples were negative the following week, suggesting either that the positive readings were false positives or that there was weak or transient T cell activation. Thus, there were no cell-mediated T cell responses detectable in peripheral blood, a result in agreement with the lack of local inflammation. Pos, positive (>50 SFUs per million cells plated) and at least threefold the medium-only control; Neg, negative (<50 SFUs per million cells plated) or less than threefold the medium-only control; Bkg, high background/not interpretable (medium control >100 SFUs per million cells plated).

Subject	Antigen	d0/FOd0	FO week 1	Week 2/FO week 2	FO week 3	Week 4/FO week 4	FO week 5	FO week 6	FO week 7	FO week 8	d90/FOd90
CH12	AAV	Neg/Neg	Neg	Neg/Neg	Neg	Neg/Neg	Neg	Neg	Neg	Neg	Neg*/Neg
	RPE65	Neg/Neg	Neg	Neg/Neg	Neg	Neg/Neg	Neg	Neg	Neg	Neg	Neg*/Neg
CH11	AAV	Neg/Bkg	Bkg	Neg/Bkg	Bkg	Neg†/Bkg	Bkg	Pos†	Neg	Neg	Neg/Neg
	RPE65	Neg/Bkg	Bkg	Neg/Bkg	Bkg	Neg†/Bkg	Bkg	Pos†	Neg	Neg	Neg/Neg
NP01	AAV	Neg/Neg	Neg	Neg/Bkg	Neg	Neg/Bkg	Neg	Neg	Bkg	Neg	Neg/Bkg
	RPE65	Neg/Neg	Neg	Neg/Bkg	Neg	Neg/Bkg	Pos†	Neg	Bkg	Neg	Neg/Bkg

^*Poor viability of cells.

^†Positive result likely due to high background reactivity.