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. 2014 Aug 14;5:26. doi: 10.1186/2041-9139-5-26

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Copyright © 2014 Peres et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Cosinor curves for the relative mRNA expression of Clock Genes in Nematostella vectensis. Data of a)Clock, b)Cycle, c)Timeout, d)Cry1a, e)Cry1b and f)Cry2 in individuals of Nematostella vectensis kept in a light–dark (12:12 h), constant darkness cycle, or constant darkness with melatonin supplementation (0.1 μM) normalized by the ribosomal protein P0, where a significant curve fit was obtained. The horizontal line, the mesor, represents the mean oscillation, and the vertical arrow, the acrophase, the time of occurrence of the daily-peak of melatonin. Each point represents five individuals/samples.