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. Author manuscript; available in PMC: 2014 Sep 19.
Published in final edited form as: Nanomedicine (Lond). 2013 Jul 9;9(2):221–235. doi: 10.2217/nnm.13.100

Figure 4. Immunofluorescence imaging of the spleen after potato virus X administration and colocalization with cellular markers.

Figure 4

Immunohistological analysis of spleen sections 24 and 72 h postintravenous administration of A647-PVX-PEG in Balb/c mice. (A1 & B1) are the fluorescence microscopic image of whole spleen section stained for PVX (green), B220 marker (magenta) and nuclei (4′,6-diamidino-2-phenylindole) after 24 and 72 h, respectively; (A2–A4 & B2–B4) are the corresponding confocal images from the regions of the same spleen. (C1 & D1) are the spleens similarly stained for CD223 (magenta) at 24 and 72 h (C2–C4 & D2–D4) with corresponding confocal images. (E1 & F1) are the spleens stained for CD3 (magenta), (E2–E4 & F2–F4) are the corresponding confocal images. Colocalization analysis was carried out using ImageJ software and is shown in yellow; M2 coefficients are reported in Table 1. Scale bars are 1000 μm for (A1–F1) and 50 μm for all confocal images.

PEG: Polyethylene glycol; PVX: Potato virus X.