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. Author manuscript; available in PMC: 2015 Jul 2.
Published in final edited form as: Neuron. 2014 Jul 2;83(1):189–201. doi: 10.1016/j.neuron.2014.05.018

Figure 4. In Vitro Optogenetic Inhibition of the DG and CA3 in ArcCreERT2 x R26R-CAG-STOP-floxed-Arch-GFP Mice.

Figure 4

(A) Genetic design.

(B) Representative Arch-GFP+ DG cell.

(C and D) In vitro photostimulation (500 ms, yellow bar) of DG cells resulted in 162.49 ± 38.26 pA steady-state current in Arch-GFP+ neurons (n = 18). This corresponds to −13.1 ± 2.5 mV hyper-polarization, when the cells were current clamped at their resting potentials (−73.47 ± 1 mV) (n = 18).

(E) Photostimulation-evoked hyperpolarization was able to abolish current injection-induced action potentials (APs). Example traces represent 150 pA somatic current injection without or with light stimulation (n = 5).

(F) Arch photostimulation was able to abolish trains of current injection-evoked APs.

(G) The graph represents the increasing amount of steady-state current as a function of laser power (n = 3).

(H) Representative Arch-GFP+ CA3 neuron.

(I and J) In CA3, in vitro photostimulation also resulted in complete inhibition of APs. Voltage (upper trace) and current clamp (lower trace) recording of an Arch-GFP+ CA3 neuron. In vitro photostimulation resulted in 408.23 ± 54.79 pA steady-state current in all of the Arch-GFP+ CA3 neurons, which corresponds to −25.93 ± 6.66 mV hyperpolarization (n = 3). Error bars represent ± SEM.