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. Author manuscript; available in PMC: 2014 Sep 19.
Published in final edited form as: Cell Immunol. 2013 Dec 18;287(2):78–85. doi: 10.1016/j.cellimm.2013.12.003

Fig. 4.

Fig. 4

Effect of deprivation of either L-serine or L-serine and glycine on the [3H]TdR incorporation into DNA of G0 T cells following immobilized anti-CD3 activation. Murine G0 T cells were activated with immobilized anti-CD3 for 40 h in the complete RPMI 1640 medium (control) or the RPMI 1640 medium derived of either L-serine or L-serine and glycine. These culture media were purchased from JBI (Daegu, Korea), and supplemented with 10% dialyzed FBS (JRH Biosciences, KS, USA). For [3H]TdR incorporation assay, the last 2 h was pulsed with 1 μCi of [3H]TdR before the cells were harvested. Each value is expressed as mean ± SD (n = 3). *P < 0.05 as compared with the control.