Notch is expressed in the abdominal appendage. a At 48 h APF, Notch is strongly expressed in the developing joint (line), the body of the appendage, and the bristle cells (bc). b A DAPI image of a. c An overlay of a and b shows the strong expression of Notch in the joint region compared to the number of nuclei. d A close-up of the bristles shows Notch expression (red) occurs in the bristle shaft cell, but not in the nucleus of that cell (bcn, blue). Expression of 22c10 (e) labels both the bristle shaft cell and the bristle neuron. f a summary of Notch expression in the abdominal appendage